Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation

Baharlou, Rasoul and Ahmadi-Vasmehjani, Abbas and Faraji, Fatemeh (2017) Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation. INTERNATIONAL IMMUNOPHARMACOLOGY, 47. pp. 59-69.

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Abstract

Abstract Background and objectives: Mesenchymal stem cells (MSCs) are multipotent adult stem cells with immunomodulatory properties. The mechanisms by which MSCs inhibit the proliferation of pro-inflammatory T cells have not been fully elucidated yet. It is assumed that pro-inflammatory T-cells play an important role in the development of autoimmune diseases. We investigated the potential therapeutic effects of human adipose tissue derived (Ad)-MSCs on the peripheral blood mononuclear cells (PBMCs) of rheumatoid arthritis (RA) patients and healthy individuals, with a particular focus on Th17-associated cytokines. Materials and methods: PBMCs from RA patients and healthy donors were co-cultured with Ad-MSCs and HeLa with or without Phytohemagglutinin (PHA). Finally, IL-6, IL-17, IL-21, IL-23 and TGF-beta levels were determined by ELISA and quantitative real-time RT-PCR on co-culture supernatants and PBMCs, respectively. Results: In co-culture interaction, Ad-MSCs inhibited IL-17 secretion by PBMCs compared to unstimulated PBMCs cultured alone. In addition, IL-21 expressions in PBMCs of the patient group, and IL-17 and IL-21 in healthy group were inhibited by Ad-MSCs compared to PBMCs cultured alone. TGF-beta expression in healthy individuals remarkably increased in both MSC-treated groups with and without PHA in comparison to PHA-stimulated and -unstimulated PBMCs. Conclusions: This study demonstrates that human Ad-MSCs act as key regulators of immune tolerance by inhibiting the inflammation. Therefore, they can be attractive candidates for immunomodulatory cell-based therapy in RA. (C) 2017 Published by Elsevier B.V. KEYWORDS: Adipose, tissue-derived, mesenchymal

Item Type: Article
Subjects: QW Microbiology and Immunology
Divisions: School of Medicine
Depositing User: mrs Maryam azmoode
Date Deposited: 16 Aug 2017 06:29
Last Modified: 16 Aug 2017 06:29
URI: http://eprints.jums.ac.ir/id/eprint/57

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